Module 3B Calculating the molar absorbance coefficient from some data
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Module 3B Calculating the molar absorbance coefficient from some data.
Given the data below, calculate the molar absorbance coefficient. The width of the cuvette was 1 cm.
Concentration (mM)

Absorbance

2

0.16

5

0.39

10

0.78

15

1.16

20

1.54

epsilon = 0.08 mM^{1}.cm^{1}
= 80 M^{1}.cm^{1}
express your answer to one significant figure.
well done
incorrectchoose two points on the line (10, 0.078) (20, 1.54)slope = (1.540.078) / (2010)= 0.76 / 10= 0.08 mM^{1}the pathlength was 1cm so epsilon = 0.08 mM^{1}.cm^{1} = 80 M^{1}.cm^{1}
Given the data below, calculate the molar absorbance coefficient. The width of the cuvette was 1 cm.
Concentration (μM)

Absorbance

2

0.12

4

0.25

6

0.35

8

0.49

10

0.59

epsilon = 0.06 μM^{1}.cm^{1}
= 60000 M^{1}.cm^{1}
express your answer to one significant figure.
well done
incorrectchoose two points on the line (10, 0.078) (20, 1.54)slope = (0.590.12) / (102)= 0.47 / 8= 0.06 μM^{1}the pathlength was 1cm so epsilon = 0.06 μM^{1}.cm^{1} = 60000 M^{1}.cm^{1}
Given the data below, calculate the molar absorbance coefficient. The width of the cuvette was 1 cm.
Concentration (μg/L)

Absorbance

10

0.039

20

0.077

40

0.157

80

0.310

150

0.586

epsilon = 0.004 μg^{1}.L.cm^{1}
= 4000 M^{1}.L.cm^{1}
express your answer to one significant figure.
well done
incorrectchoose two points on the line (10, 0.039) (20, 0.077)slope = (0.0770.039) / (2010)= 0.038 / 10= 0.04 μg^{1}.Lthe pathlength was 1cm so epsilon = 0.04 μg^{1}.L.cm^{1} = 4000 g^{1}.L.cm^{1}