1

Given the data below, calculate the molar absorbance coefficient. The width of the cuvette was 1 cm.

epsilon = mM^{-1}.cm^{-1} = M^{-1}.cm^{-1} express your answer to one significant figure.

incorrect

choose two points on the line (10, 0.078) (20, 1.54)

slope = (1.54-0.078) / (20-10)

= 0.76 / 10

= 0.08 mM^{-1}

the pathlength was 1cm so epsilon = 0.08 mM^{-1}.cm^{-1} = 80 M^{-1}.cm^{-1}

2

epsilon = μM^{-1}.cm^{-1} = M^{-1}.cm^{-1} express your answer to one significant figure.

slope = (0.59-0.12) / (10-2)

= 0.47 / 8

= 0.06 μM^{-1}

the pathlength was 1cm so epsilon = 0.06 μM^{-1}.cm^{-1} = 60000 M^{-1}.cm^{-1}

3

epsilon = μg^{-1}.L.cm^{-1} = M^{-1}.L.cm^{-1} express your answer to one significant figure.

choose two points on the line (10, 0.039) (20, 0.077)

slope = (0.077-0.039) / (20-10)

= 0.038 / 10

= 0.04 μg^{-1}.L

the pathlength was 1cm so epsilon = 0.04 μg^{-1}.L.cm^{-1} = 4000 g^{-1}.L.cm^{-1}