6.7 Genotyping of specific mutations

To determine which version of a mutation or polymorphism an individual has allele-specific PCR is used. In this method one primer is common to both reactions and anneals close to the mutation. There are two versions of the other primer. These are allele specific and anneal to the variation. The 3' end of the allele-specific primer is modified to only anneal to one of the alleles. For example:

Figure showing how the allelic genotype of an organism can be elucidated.
Figure 22: Genotyping using PCR
Figure showing how the allelic genotype of an organism can be elucidated.
Figure 23: Genotyping using PCR: table
© SCBC 2007