Design your own PCR program

Cells_selfassessment

Design your own PCR programme using the following primers to amplify a DNA fragment of 1.8 Kb. The reaction is set up in the evening and will be collected the following day. Fill in your own answers, then see if the data matches the answer in the Display Answer below.

Primers

Primer A GTACTTGACTGGCATGAC Annealing Temp = C

Primer B TAGGTAGCAATCGTGAGC Annealing Temp = C

Step 1 C for min.

Step 2 C for min.

Step 3 C for min.

Step 4 C for min.

Step 5 C for min.

Step 6 C for .

Step 7 C for min.

Repeat Steps 2 - 4 for 25-30 cycles.


Question 1

Design your own PCR programme using the following primers to amplify a DNA fragment of 1.8 Kb. The reaction is set up in the evening and will be collected the following day.

© SCBC 2007